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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Chem Biol Interact. 2011 Jan 15;190(1):62–72. doi: 10.1016/j.cbi.2011.01.009

Figure 3. ‘Stemness’ marker protein expression is reduced in hepatic-like cells.

Figure 3

Passage-matched hESCs were cultured in parallel on either hFF feeder layers in hESC media (hESCs) or on collagen in hepatocyte media (Hepatic-like cells). At 10 days in culture cells were probed for OCT4, SOX2, SSEA-3, TRA-1-60, and TRA-1-81 protein expression by immunofluorescence using antibodies specified in the Methods. Cells were examined microscopically and phase-contrast and fluorescence images were captured.