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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Chem Biol Interact. 2011 Jan 15;190(1):62–72. doi: 10.1016/j.cbi.2011.01.009

Figure 7. Hepatic-like cells exhibit the hepatic-specific functions of ICG uptake and glycogen storage.

Figure 7

Passage-matched hESCs were cultured in parallel on either hFF feeder layers in hESC media (hESCs) or on collagen in hepatocyte media (Hepatic-like cells) for either 10 or 20 days. Cells were evaluated for ICG uptake (cyan-green) by incubation with 100 μg/ml ICG and for glycogen storage capacity (pink) using periodic acid-Schiff (PAS) staining, both as described in Materials and Methods. Cells were examined microscopically and phase-contrast images were captured. Cultures of adult primary human hepatocytes (Hepatocytes) assessed in parallel were included as positive controls.