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Representative examples of protein tyrosine nitration (total 3-nitrotyrosine immunoreactivity) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in quadriceps of control subjects, severe scoliosis patients and chronic obstructive pulmonary (COPD) patients. Several tyrosine-nitrated proteins were detected. Monoclonal anti-GAPDH antibody was used to control equal loading among various lanes. MW: molecular weight.
