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. 2010 Nov 1;7(6):803–811. doi: 10.4161/rna.7.6.13484

Figure 1.

Figure 1

Mss116p-promoted splicing of the ai5γ intron in yeast mitochondria. (A) Comparison of the in vivo splicing activity in a wt yeast strain and a mss116-knockout strain. In the presence of Mss116p 70–75% of the cox1 pre-RNA are spliced, while less than 1% of splicing occurs in the absence of Mss116p. (B) Scheme of the Poison Primer Assay used to assess in vivo splicing activities. A primer hybridizes to the 5′ end of the downstream exon and is extended by a Reverse Transcriptase in the presence of ddCTP (and dATP, dGTP, dTTP). The extension therefore stops at the first G in the template, which results in a Primer + 14 nts product for the ligated exons and Primer + 8 nts for the unspliced pre-RNA.