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Addition of a heterologous trimerization domain to the RNA chaperone region of N abrogates RHD activity. N Δ175–313 contains the intrinsic trimerization domain of N and N 175F contains the foldon trimerization domain. RNA chaperone (RHD) activity was determined as in Figure 4. Data for the RHD activity of N-His175F are also included in the graph but not visible as RHD activity was undetectible. All assays were performed a minimum of three times.
