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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Eur J Immunol. 2010 Oct;40(10):2891–2901. doi: 10.1002/eji.201040485

Figure 2. Phenotypic characterization of CD8+ T cells from healthy control subjects after 5 days of culture with anti-CD3 mAb.

Figure 2

(A) PBMC were gated on CD8+ cells and stained for CD25, CD127, CTLA4, GITR, and FoxP3. A representative experiment of 4–8 independent experiments is shown. (B, C) PBMC cultured with anti-CD3 for 5 days were washed, rested in the absence of the mAb for three days, and then restimulated with PMA and ionomycin (C) or left untreated (B) for 6 hours in the presence of Golgi block. The cells were subsequently stained for intracellular cytokines and analyzed by flow cytometry. The plots show gated CD8+ populations with quadrant tool placed on the basis of isotype controls. The percentage of the gated cells is shown in each quadrant. A representative of 3 separate experiments with similar results is shown. (D) Comparison of relative numbers of FoxP3+ CD4+ (left) and CD8+ (right) cells 5 days after incubation with anti-CD3; a representative experiment out of 4 is shown. The percentage of cells within the indicated region (set based on isotype control) are shown.