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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Autophagy. 2008 Apr 7;4(5):659–668. doi: 10.4161/auto.6058

Figure 1.

Figure 1

Combined inhibition of caspase-3 and mTOR reduces H460 lung cancer cells clonogenic survival. H460 NSCLC cells were treated with DMSO control, Z-DEVD (50 μM, for 24 hrs), RAD001 (10 nM, for 1 hr) or Z-DEVD and RAD001 with radiation. After 8 days, colonies were stained and the scored colonies were graphed. Survival curves for H460 cells ± Z-DEVD, mTOR or Z-DEVD and RAD001 treatment. The DER was calculated for each of the conditions with comparison to the DMSO control group. The Z-DEVD, RAD001 and combined Z-DEVD and RAD001 treatment groups had DERs of 1.1 (p < 0.01), 1.28 (p < 0.009) and 1.53 (p < 0.009), respectively. All statistical analyses were performed using Student’s t-test with comparison to the DMSO control group. Points, mean of three independent experiments; bars, S.D.