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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Autophagy. 2008 Apr 7;4(5):659–668. doi: 10.4161/auto.6058

Figure 4.

Figure 4

Z-DEVD/RAD001 sensitizes vascular endothelial in vitro model to ionizing radiation and reduces in vivo vascular density in irradiated H460 tumors. (A) Representative histological section following von Willebrand factor (vWF) staining. Histologic sections were obtained from the tumors of the mice in each treatment group from the in vivo tumor volume study, and stained for blood vessels using an antibody for vWF. (B) Blood vessels were quantified by randomly selecting 400X fields and counting the number of blood vessels per field. This was done in triplicate and the average of the three counts was calculated. Columns, mean number of vessels counted per microscopic field; bars, S.D. (C) Human umbilical vein endothelial cells (HUVECs) were treated with 5 μmol/L Z-DEVD/RAD001 and then immediately irradiated with either 0 or 5 Gy. Six hours later, cells were trypsinized and re-plated on 24-well plates coated with Matrigel. After 24 hrs, cells were fixed and stained with H&E. The slides were examined by microscopy (×100), and representative fields are shown. (D) Stained tubules were then counted in three separate, randomly selected fields. Columns, mean number of tubules counted per microscopic field; bars, S.D.