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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Autophagy. 2008 Apr 7;4(5):659–668. doi: 10.4161/auto.6058

Figure 5.

Figure 5

Z-DEVD/RAD001 with radiation increased autophagy induction in H460 tumor model. (A) GFP-LC3-transfected H460 cells were treated with either DMSO control, Z-DEVD (50 μM, for 24 hrs), RAD001 (10 nM, for 1 hrs) or Z-DEVD + RAD001 with 0 or 5 Gy radiation, and subsequently examined by fluorescence microscopy after 48 hrs. The percentage of cells with punctate GFP-LC3 fluorescence was calculated relative to all GFP-positive cells. Columns, mean percentage of GFP-LC3 expressing punctate cells in 3 microscopic fields; Bars, S.D. (B) Representative electron micrograph image showing autophagic vacuoles with content (black arrows) following Z-DEVD/RAD001 treatment with radiation in an in vivo H460 xenograft model. (C) H460 cells were treated with Z-DEVD + RAD001 + 5 Gy. They were then treated with either 3MA (200 uM) or pepstatin A (10 μg/ml) and E64d (10 μg/ml) for 2 hrs. Cells were harvested in one or two days for western analyses for LC3-II.