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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Autophagy. 2008 Apr 7;4(5):659–668. doi: 10.4161/auto.6058

Figure 6.

Figure 6

Inhibition of autophagy by targeting siRNA to ATG5 and Beclin-1 confers radioresistance in H460 lung cancer cells. (A) ATG5/Beclin-1 expression was determined by Western blotting using lysates from H460 lung cancer cells treated with siRNAs against ATG5/Beclin-1. Actin was probed to demonstrate equal loading. (B) H460 lung cancer cells were treated with DMSO control, siRNA control and siRNA ATG5/Beclin-1 (12.5 nM) under irradiation with doses ranging from 0 to 6 Gy. After 8 days, colonies were stained and the scored colonies were graphed. The DER in H460 cells treated with siRNA ATG5/Beclin-1 was 0.85 (p = 0.021, Student’s t-test). Points, mean of 3 independent experiments; bars, S.D.