Figure 5.

Evaluation of Aspergillus virulence in Toll-deficient Drosophila flies. (A) Survival rates in Tlr⁶³²/Tl^I-RXA flies following injection of the hypovirulent ΔgliP Aspergillus strain or its isogenic WT strain AF293. (B) Survival rates in Tlr⁶³²/Tl^I-RXA flies eight days after infection with the hypovirulent Δalb1 Aspergillus strain compared with infection with its isogenic WT strain B-5233 in the three infection assays. (C) qPCR analysis of the tissue fungal burden in Tlr⁶³²/Tl^I-RXA flies infected with Δalb1 Aspergillus or its isogenic WT strain in the rolling assay. CE, conidial equivalent of Aspergillus fumigatus DNA.