Table 1.

Primers to make CDA1 deletion cassette and confirm gene replacement

	Primer	Sequence1
a2	CDA1-F	cac gtt tga ttg cga acg ct
b	CDA1-R	ccg acc taa gac gat cat
c	CDA1URA5-F	cca gaa gcg gaa ttt cgc cct tga tcA TGT CCT CCC
d	CDA1URA5-R	GGG AGG ACA Tga tca agg gcg aaa ttc cgc ttc tgg
e	URA5CDA1-F	CGG TGT TCA GAG GTC TTA Atc taa cga tcg atg ggg t
f	URA5CDA1-R	Acc cca tcg atc gtt aga TTA AGA CC T CTG AAC ACC G
g	CDA1-F4	atc ggt cga cag tag cat
h	CDA1-R5	gga aga aga gtc aga ggt
i	CDA1-TAA	tta ggc gac ata tac cat acc gac cgc a
j	CDA1-F5	ggt gac att gat gac cgt
k	CDA1-F3	Cca ggc ata cgt tca agt
l	URA-R	CC T CGA TGA AGG CAA CTT
m	URA-F	AGC TGC AGG AGG TCA GGA A
n	CDA1-R3	ctg tcc aca gtg cat caa

¹Lower case designates CDA1 sequence and upper case URA5 sequence.

²Primer locations are the same or similar to those designated in Figure 1B if the CDA1 gene was substituted for MP98(CDA2).