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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Microbiol Methods. 2011 Feb 23;85(2):103–113. doi: 10.1016/j.mimet.2011.02.001

Figure 2. Verification of the C-terminal epitope tagging by diagnostic PCR.

Figure 2

Confirmatory PCR was performed using genomic DNA as the template from wild type and transfected parasite clones. PCR products obtained at the 5′ junction from (A) primers DP1::DP2; (B) DP1::DP3; and at the 3′ junction of the altered chromosome with primers; (C) DP4::DP5; and (D) DP4::DP6. Lane 1: positive clone B6, Lane 2: positive clone C4, Lane 3: negative clone B7, Lane 4: wild type RH genomic DNA, Lane 5: p25.m01787MSG plasmid, Lane 6: No DNA control, and Lane 7: DNA ladder.