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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Microbiol Methods. 2011 Feb 23;85(2):103–113. doi: 10.1016/j.mimet.2011.02.001

Figure 3. Immunoblot demonstrating expression of epitope tagged ORF25.m01787.

Figure 3

Parasite lysates from wild type RH and chloramphenicol resistant parasite clones were analyzed by immunoblot using (A) peroxidase conjugated HA peptide specific monoclonal antibody; and (B) tubulin specific antibody (control antibody). The C-terminal 3HA tagged ORF25.m01787 was detected in the lysates of clones B6 and C4 (positive clones), but not in the lysates of either RH wild type or clone B7 (negative clone). The tubulin blot staining demonstrates equal loading in all lanes.