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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Microbiol Methods. 2011 Feb 23;85(2):103–113. doi: 10.1016/j.mimet.2011.02.001

Figure 4. Immunofluorescence (IFA) of clones B6 and B7 using anti HA antibody.

Figure 4

Phase, DAPI (DNA, blue), HA (red) and merged (blue and red) images of B6 (HA-positive) and B7 (HA-negative) transfectants. ORF25.m01787 is predicted to be a homolog of RNA polymerase III transcription factor and expected to localize to the nucleus. As demonstrated B6 exhibits specific nuclear location. The absence of antibody specific signal in clone B7 proves the specificity of the antibody (HA signal is overexposed in the HA panel of B7 illustrating that nuclear localization is not present; merged panel shows similar exposures of DAPI and HA for B6 and B7).