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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Microbiol Methods. 2011 Feb 23;85(2):103–113. doi: 10.1016/j.mimet.2011.02.001

Figure 5. Homologous integration of the epitope tagged PCR product at the target gene locus.

Figure 5

Position of the target gene specific (open double sided arrow) and CAT gene (striped double sided arrow) specific probes in the genomic context have been depicted in the wild type (Fig A) and positive transformant (Fig B). Southern blot analysis of the BglII and EcoRV plus Sac1 (RV+S) digested genomic DNA from wild type RH, positive parasite clone B6 and negative clone B7. Blots were sequentially probed with DIG labeled gene specific (open double headed arrow) and CAT (chloramphenicol) selection cassette specific (striped double headed arrow) PCR probes (Fig C).