Table 1. Primer Sequences.
Restriction enzyme sequences are underlined, attB sequences are indicated by bold stype
| RFC.1Nsi1For | GGCCATGCATCAAACAAGTTTGTACAAAAAAGCTGAACG |
|---|---|
| RFC.1NheRev | CAGGCTAGCCAATCGAACCACTTTGTACAAGAAAGC |
| Kpn1BRF1For | ATCGGTACCTGCAGGAACAAATTCCCGTATACGCGAG |
| BRF1NheRev | GTAGCTAGCGTTCCCGTTTTGCTCAGTGACTCTTCGTACAGG |
| Pac1BRF1For | CGTTAATTAAAAGAGTAGCAGACTCTGTGACGAGCGAAGACC |
| BRF1SacIIRev | CGACCGCGGAGTCAAATTGCTGAGGCCTCGCAGGACAGTGCG |
| attB4BRF1For | GGGGACAACTTTGTATAGAAAAGTTGTCTGCAGGAACAAATTCCCGTATACGCGAG |
| attB1BRF1Rev | GGGGACTGCTTTTTTGTACAAACTTGCAGTCAAATTGCTGAGGCCTCGCAGGACAGTGCG |
| attB2BRF1For | GGGGACAGCTTTCTTGTACAAAGTGGCGTCTGCTCAACACAGAGTGTACAACCGG |
| attB3BRF1Rev | GGGGACAACTTTGTATAATAAAGTTGCCAAGAGCCTCTCGCTGGAGTTCCAGG |
| attB1CATFor | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCGATAAGCTTGATGGCGATGCATGTCC |
| attB2CATRev | GGGGACCACTTTGTACAAGAAAGCTGGGTCGTGTTAACCGGTTCGACTAAAACAAC |
| BRF1HAIntFor | GATGCTTTCTGGTTATCCGAGCTTATGTCGACTTCAGCG |
| BRF1HAIntRev | GTTGACCATTGATCTACCGTACTTTGTCCG |
| DP1 | CACTTGGTCCTTTGCAGAATTTTGTACGCCC |
| DP2 | CATCATATGGATAGCTAGCGTTCCCGTTTTGC |
| DP3 | GGACATGCATCGCCATCAAGCTTATC |
| DP4 | CTTGTTGTGCTTCTGTCAAGCACCTCGGC |
| BRF1DP5 | CTGGAT ATA CCACCGTTGATATATCCC |
| BRF1DP6 | TCTTCGCCCCCGTTTTCACCATGGG |
| attB4UPRT2kbFor | GGGGACAACTTTGTATAGAAAAGTTGTCCGTGTACCTGCCGGGCCTTGCATCC |
| attB1UPRT2kbRev | GGGGACTGCTTTTTTGTACAAACTTGCCGTAGAAGCCGGGGCCGCTACAAGG |
| attB2UPRT2kbFor | GGGGACAGCTTTCTTGTACAAAGTGGCGGACAGACCGCTGACGGAATCGCGG |
| attB3UPRT2kbRev | GGGGACAACTTTGTATAATAAAGTTGCCCTGCCGGTGCGTTTGCGCTCTTC |
| UPRT2kbFor(DisFLF) | CTCGCCTCAGACAATTTGTCAACTGC |
| UPRT2kbRev(DisFLR) | CTGGTGCGTACTTCTGTATGTAGCC |
| UPRT1kbFor(DisFLF) | GCGCCACCCGCTGTGCCTAGTATCG |
| UPRT1kbRev(DisFLR) | CGGGAATCAGACCCTCGTCTCCGGTGG |
| UPRT0.5kbFor(DisFLF) | GTGACTGATTTTCTGCACGTTGGC |
| UPRT0.5kbRev(DisFLR) | GTAACGTGGACCATTCTTCACATTGC |
| DisSplit For | CCACCGTTGATATATCCCAATGGCATCG |
| DisSplitRev | GCATTCTGCCGACATGGAAGCCATCACAAA |
| DP7 | TGCACGGATCCACAGGAGACTTTATCTCGC |
| DP8 | AATTCCCTACGGCAGGACACCGTTGTTCTTGC |
| DP9 | GCTCCTTGTCGATCCCCGATATTCGAC |
| DP10 | ATTTCGGTGACCGGTACTTTGGAACCATG |
| DP11 | CCACCGTTGATATATCCCAATGGCATCG |
| DP12 | GCATTCTGCCGACATGGAAGCCATCACAAA |
| Brf1ProbeFor | ACGCAGCTCCCTCGAAAAGGAAGGG |
| Brf1ProbeRev | AACGGTCGAGGGAACTCGAAACGCC |
| CATProbeFor | CCACCGTTGATATATCCCAATGGCATCG |
| CATProbeRev | GCATTCTGCCGACATGGAAGCCATCACAAA |
| UPRTRTFor | GCGCAGGTCCCAGCGAGCGGAAAG |
| UPRTRTRev | CATGGTTCCAAAGTACCGGTCACCG |
| Actin RTFor | GCGGATGAAGAAGTGCAAGCCTTGG |
| ActinRTRev | GAAGCACTTGCGGTGGACGATGCTCGGG |