. Author manuscript; available in PMC: 2012 May 1.

Published in final edited form as: J Microbiol Methods. 2011 Feb 23;85(2):103–113. doi: 10.1016/j.mimet.2011.02.001

Table 2.

Summary of the AMAXA transfection condition used and the results

Epitope tagging	Amount of DNA used for transfection (ηmoles)	Emergence of chloramphenicol resistance parasites	Diagnostic PCR confirmation
	1	No	-
	2	Yes	Yes
	2.5	Yes	Yes
UPRT deletion	Amount of DNA used for transfection (ηmoles)	Emergence of 5-fluoro-2-deoxyuridine/chloramphenicol resistant parasites	Confirmation by diagnostic PCR
Length of flanking homology sequence	Amount of DNA used for transfection (ηmoles)		Confirmation by diagnostic PCR
0.5kb	3	no	-
	3.0	no	-
1.0kb	1.5	no	-
	3.0	Yes	Yes
2.0kb	1.5	No	-
	3	Yes	Yes