Skip to main content
. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Biochem Biophys Res Commun. 2005 Jun 24;332(1):58–67. doi: 10.1016/j.bbrc.2005.04.093

Figure 2. Effect of tyrosine kinase inhibitors on PLD2.

Figure 2

Transfected COS-7 cells were cultured continuously with genistein for 48 hours. Cells were harvested and cell lysates (~0.8 mg protein/ml) were immunoprecipitates with anti-myc antibodies that were used for measuring enzymatic activity (A) or immunoblotting (B). In (C) the effect of the EGF-R inhibitor AG-18 was assayed n vitro in the presence or assensu of PTP1B. Both activity and immunoblotting (inset) were measured.