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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Biochem Biophys Res Commun. 2005 Jun 24;332(1):58–67. doi: 10.1016/j.bbrc.2005.04.093

Figure 6. PTP1B increases tyrosine phosphorylation of PLD2 in a stable cell line.

Figure 6

(A) Stable COS-7 cells were harvested and cell lysates (~0.8 mg protein/ml) were treated with the indicated concentrations of recombinant PTP1B, immunoprecipitated with anti-myc antibodies and used for SDS/Western blotting developed with anti-phosphotyrosine antibodies (clone PY100). (B) COS-7 cell lysates treated with the indicated concentrations of PTP1B were immunoprecipitated with anti-myc antibodies. Immunocomplex beads were run in SDS/PAGE gels and resulting blots were developed with anti-PY antibodies. The arrow marks the position a heavily Tyr-phosphorylated protein with an apparent Mw of 29 kDa.