Table 1.
Effect of concentration of cell line-lysate on encapsulation profile. Differing numbers of cells were deliberately dissolved within a finite volume of PBS. When keeping PLGA MW constant, extent of encapsulation increased with increasing concentration of lysate.
| Protein concentration of lysate (mg/ml) | PLGA MW (K) | Protein encapsulated (μg/mg) | Efficiency of encapsulation (%) |
|---|---|---|---|
| 60 | 45 | 40 | 66.7 |
| 50 | 45 | 26 | 52 |
| 25 | 45 | 21.2 | 84.8 |
| 12.5 | 45 | 6 | 48 |
| 6.25 | 45 | 5.4 | 88.2 |
| 60 | 80 | 46 | 76.7 |
| 50 | 80 | 36 | 72 |
| 25 | 80 | 26 | ≈100 |
| 12.5 | 80 | 6.6 | 52.8 |
| 6.25 | 80 | 5.4 | 85 |