. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: Nanomedicine. 2010 Aug 6;7(1):1–10. doi: 10.1016/j.nano.2010.07.002

Table 4.

Effect of lyophilization of fresh tumor-derived lysate on encapsulation. Limited availability of fresh tumor material resulted in a relatively dilute lysate. However lyophilization enabled increase in the extent of encapsulation.

Protein concentration of lysate (μg/μl)	Volume of lysate (μl)	Type of lysate^*	Protein content of starting lysate (μg)		Weight of NP used for encapsulation (mg)	Protein content of NP (μg/mg)	Encapsulation efficiency (%)
32.5	50	S	1625		50	14	43.1%
23	100	L	2300		50	19.6	42.6%

S= Soluble; L=Lyophilized