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. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: J Mol Biol. 2011 Feb 19;408(1):147–162. doi: 10.1016/j.jmb.2011.02.026

Figure 2.

Figure 2

Two-dimensional gel electrophoresis of an E. coli lysate after pulse proteolysis in 3.0 M urea with and without ATPγS. An E. coli cell lysate was incubated with 1.0 mM ATPγS in 3.0 M urea for 2 hrs before pulse proteolysis. A control sample was also prepared under the identical condition except without 1.0 mM ATPγS. Spots with different intensities between the two gels were identified. Spots selected for in-gel digestion are labeled by numbers. Spots 1 and 2 are distinguishable on the actual gel but look as a single spot on this image. Two spots, 11 and 12, only appeared in the control gel. The major spot near 36.5 kDa corresponds to thermolysin.