Table 1.

Identified proteins

Spot	Gene namea	Descriptiona	ATP binding
			Gene Ontologyb	Experimental Evidencec	Kd (μM)
1	atpA	ATP synthase, F1 complex, α subunit	√	√	0.140
2	lpd	Lipoamide dehydrogenase (E3 monomer)
3d	glnS	Glutaminyl-tRNA synthetase	√	√	19058
4,7d	pheS	Phenylalanyl-tRNA synthetase, α-chain	√	√	60059
5	pfkA	6-phosphofructokinase-1	√	√	0.02560
6	yncE	Hypothetical protein	√
8	sucD	Succinyl-CoA synthetase, α subunit	√	√	9061
9	mlaC	Periplasmic binding protein of the phospholipid ABC transporter
10	groS	GroES	√	√44
11,12d	gapA	Glyceraldehyde-3-phosphate dehydrogenase-A

^aThe gene name and the description of each protein were collected from EcoCyc, E. coli functional genomics database.

^bGenes annotated with the GO term ‘ATP binding’ (GO:0005524) in EcoCyc database.

^cProteins whose ATP binding is confirmed experimentally.

^dThe spot was found to contain a fragment of the identified protein.