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. Author manuscript; available in PMC: 2012 Mar 22.
Published in final edited form as: Curr Biol. 2011 Mar 22;21(6):449–459. doi: 10.1016/j.cub.2011.02.019

Figure 1. Stage-by-stage small RNA replicates.

Figure 1

Scatter plots depict a gene-by-gene comparison of small RNA abundance. Each plot compares data from ego-1(+) and ego-1(om84) animals at a single developmental stage. Each point represents a single gene, with an (x,y) coordinate defined by raw (non-normalized) counts of corresponding antisense small RNAs from the indicated libraries. A black line shows the ratio of total counts in each pair of samples giving the expected parity between samples. Two median lines are shown for each dataset: (i) median of the ratio of ego-1(om84)/ego-1(+) on a gene-by-gene basis (ii) median of the ratio of ego-1(om84)/ego-1(+) on a gene-by-gene basis using only those genes for which the sum of ego-1(om84) and ego-1(+) counts is greater than 200. Genes whose small RNA abundance increases [blue] or decreases [red] 3-fold (2-fold in L3) in ego-1(om84) (posterior probability ratio (PPR) < 0.005) in an independent replicate of the specified stage. (A) L3. (B) L4. (C) Adult.