FIG. 1.

Characterization of isolated human-exfoliated deciduous teeth (SHED). (A) Schematic timeline of the procedures conducted for this study. (B) Exfoliated deciduous incisor containing dental pulp. Cultured SHED had a fibroblast-like morphology (C) and expressed STRO-1 (D), CD146 (E), CD90 (F), CD29 (G), vimentin (H), DSP (I), nestin (K), and βIII-tubulin (L), but were negative for CD34 (J), control (M). (N) Flow cytometry analysis of SHED. Numbers in panels represent mean fluorescent intensity of the cells expressing each marker. Scale bars: 20 μm (C–E, G, I–K, and M) and 10 μm (F, H, and L).