TABLE 1.
Profiling of free sterols from Arabidopsis
| Compound number | Sterol lipida | Formula (M)b | (M + betainyl)(m/z)c | Product ion FB(m/z) |
|---|---|---|---|---|
| 1 | Cholesterol | C27H46O | 486.4311 | 118.0859 |
| 2 (I.S.) | Cholestanol | C27H48O | 488.4468 | 118.0859 |
| 3 | Campesterol | C28H48O | 500.4468 | 118.0859 |
| 4 | Stigmasterol | C29H48O | 512.4468 | 118.0859 |
| 5 | Sitosterol | C29H50O | 514.4624 | 118.0859 |
| 6 (I.S.) | Stigmastanol | C29H52O | 516.4781 | 118.0859 |
I.S., internal standard.
a
Stigmasterol and isofucosterol were not distinguished by Q-TOF measurements.
b
Formula of the parental, non-charged molecule.
c
Molecular weight of the cation formed of the parental molecule after derivatization with N-chlorobetainyl chloride.