TABLE 2.
Profiling of sterol esters from Arabidopsis
| Compound number | Sterol ester molecular speciesa | Formula (M)b | (M + NH4)(m/z)c | Product ion FS(m/z) |
|---|---|---|---|---|
| 7 | 16:3-Cholesterol | C43H70O2 | 636.5714 | 369.3505 |
| 8 | 16:2-Cholesterol | C43H72O2 | 638.5871 | 369.3505 |
| 9 (I.S.) | 16:1-Cholesterol | C43H74O2 | 640.6027 | 369.3505 |
| 10 (I.S.) | 16:0-Cholesterol | C43H76O2 | 642.6184 | 369.3505 |
| 11 | 16:3-Campesterol | C44H72O2 | 650.5871 | 383.3662 |
| 12 | 16:2-Campesterol | C44H74O2 | 652.6027 | 383.3662 |
| 13 | 16:1-Campesterol | C44H76O2 | 654.6184 | 383.3662 |
| 14 | 16:0-Campesterol | C44H78O2 | 656.6340 | 383.3662 |
| 15 | 16:3-Stigmasterol | C45H72O2 | 662.5871 | 395.3662 |
| 16 | 16:2-Stigmasterol | C45H74O2 | 664.6027 | 395.3662 |
| 17 | 16:3-Sitosterol | C45H74O2 | 664.6027 | 397.3818 |
| 18 | 18:3-Cholesterol | C45H74O2 | 664.6027 | 369.3505 |
| 19 | 16:1-Stigmasterol | C45H76O2 | 666.6184 | 395.3662 |
| 20 | 16:2-Sitosterol | C45H76O2 | 666.6184 | 397.3818 |
| 21 | 18:2-Cholesterol | C45H76O2 | 666.6184 | 369.3505 |
| 22 | 16:0-Stigmasterol | C45H78O2 | 668.6340 | 395.3662 |
| 23 | 16:1-Sitosterol | C45H78O2 | 668.6340 | 397.3818 |
| 24 (I.S.) | 18:1-Cholesterol | C45H78O2 | 668.6340 | 369.3505 |
| 25 | 16:0-Sitosterol | C45H80O2 | 670.6497 | 397.3818 |
| 26 (I.S.) | 18:0-Cholesterol | C45H80O2 | 670.6497 | 369.3505 |
| 27 | 18:3-Campesterol | C46H76O2 | 678.6184 | 383.3662 |
| 28 | 18:2-Campesterol | C46H78O2 | 680.6340 | 383.3662 |
| 29 | 18:1-Campesterol | C46H80O2 | 682.6497 | 383.3662 |
| 30 | 18:0-Campesterol | C46H82O2 | 684.6653 | 383.3662 |
| 31 | 18:3-Stigmasterol | C47H76O2 | 690.6184 | 395.3662 |
| 32 | 18:3-Sitosterol | C47H78O2 | 692.6340 | 397.3818 |
| 33 | 18:2-Stigmasterol | C47H78O2 | 692.6340 | 395.3662 |
| 34 | 20:3-Cholesterol | C47H78O2 | 692.6340 | 369.3505 |
| 35 | 18:2-Sitosterol | C47H80O2 | 694.6497 | 397.3818 |
| 36 | 18:1-Stigmasterol | C47H80O2 | 694.6497 | 395.3662 |
| 37 | 20:2-Cholesterol | C47H80O2 | 694.6497 | 369.3505 |
| 38 | 18:0-Stigmasterol | C47H82O2 | 696.6653 | 395.3662 |
| 39 | 18:1-Sitosterol | C47H82O2 | 696.6653 | 397.3818 |
| 40 | 20:1-Cholesterol | C47H82O2 | 696.6653 | 369.3505 |
| 41 | 18:0-Sitosterol | C47H84O2 | 698.6810 | 397.3818 |
| 42 | 20:0-Cholesterol | C47H84O2 | 698.6810 | 369.3505 |
| 43 | 20:3-Campesterol | C48H80O2 | 706.6497 | 383.3662 |
| 44 | 20:2-Campesterol | C48H82O2 | 708.6653 | 383.3662 |
| 45 | 20:1-Campesterol | C48H84O2 | 710.6810 | 383.3662 |
| 46 | 20:0-Campesterol | C48H86O2 | 712.6966 | 383.3662 |
| 47 | 20:3-Stigmasterol | C49H80O2 | 718.6497 | 395.3662 |
| 48 | 22:3-Cholesterol | C49H82O2 | 720.5245 | 369.3505 |
| 49 | 20:2-Stigmasterol | C49H82O2 | 720.6653 | 395.3662 |
| 50 | 20:3-Sitosterol | C49H82O2 | 720.6653 | 397.3818 |
| 51 | 20:1-Stigmasterol | C49H84O2 | 722.6810 | 395.3662 |
| 52 | 20:2-Sitosterol | C49H84O2 | 722.6810 | 397.3818 |
| 53 | 22:2-Cholesterol | C49H84O2 | 722.6810 | 369.3505 |
| 54 | 20:0-Stigmasterol | C49H86O2 | 724.6966 | 395.3662 |
| 55 | 20:1-Sitosterol | C49H86O2 | 724.6966 | 397.3818 |
| 56 | 22:1-Cholesterol | C49H86O2 | 724.6966 | 369.3505 |
| 57 | 20:0-Sitosterol | C49H88O2 | 726.7123 | 397.3818 |
| 58 | 22:0-Cholesterol | C49H88O2 | 726.7123 | 369.3505 |
| 59 | 22:3-Campesterol | C50H84O2 | 734.5401 | 383.3662 |
| 60 | 22:2-Campesterol | C50H86O2 | 736.6966 | 383.3662 |
| 61 | 22:1-Campesterol | C50H88O2 | 738.7123 | 383.3662 |
| 62 | 22:0-Campesterol | C50H90O2 | 740.7279 | 383.3662 |
| 63 | 22:3-Stigmasterol | C51H84O2 | 746.5401 | 395.3662 |
| 64 | 22:3-Sitosterol | C51H86O2 | 748.5558 | 397.3818 |
| 65 | 22:2-Stigmasterol | C51H86O2 | 748.6966 | 395.3662 |
| 66 | 22:1-Stigmasterol | C51H88O2 | 750.7123 | 395.3662 |
| 67 | 22:2-Sitosterol | C51H88O2 | 750.7123 | 397.3818 |
| 68 | 22:0-Stigmasterol | C51H90O2 | 752.7279 | 395.3662 |
| 69 | 22:1-Sitosterol | C51H90O2 | 752.7279 | 397.3818 |
| 70 | 22:0-Sitosterol | C51H92O2 | 754.7436 | 397.3818 |
I.S., internal standard.
a
Stigmasterol and isofucosterol were not distinguished by Q-TOF measurements.
b
Formula of the parental, non-charged molecule.
c
Molecular weight of the cation formed of the parental molecule with ammonium.