TABLE 4.
Profiling of acylated sterol glucosides from Arabidopsis
| Compound number | Acylated sterol glucoside molecular speciesa | Formula (M)b | (M+NH4)+(m/z)c | Product ion FS (m/z) | Product ion FAG (m/z) |
|---|---|---|---|---|---|
| 77 | 16:3-Glc-Cholesterol | C49H80O7 | 798.6242 | 369.3505 | 395.2428 |
| 78 | 16:2 Glc-Cholesterol | C49H82O7 | 800.6399 | 369.3505 | 397.2585 |
| 79 | 16:1-Glc-Cholesterol | C49H84O7 | 802.6555 | 369.3505 | 399.2741 |
| 80 | 16:0-Glc-Cholesterol | C49H86O7 | 804.6712 | 369.3505 | 401.2898 |
| 81 | 16:3-Glc-Campesterol | C50H82O7 | 812.6399 | 383.3662 | 395.2428 |
| 82 | 16:2-Glc-Campesterol | C50H84O7 | 814.6555 | 383.3662 | 397.2585 |
| 83 | 16:1-Glc-Campesterol | C50H86O7 | 816.6712 | 383.3662 | 399.2741 |
| 84 | 16:0-Glc-Campesterol | C50H88O7 | 818.6868 | 383.3662 | 401.2898 |
| 85 (I.S.) | 16:0-Glc-Campestanol | C50H90O7 | 820.7025 | 385.3818 | 401.2898 |
| 86 | 16:3-Glc-Stigmasterol | C51H82O7 | 824.6399 | 395.3662 | 395.2428 |
| 87 | 16:2-Glc-Stigmasterol | C51H84O7 | 826.6555 | 395.3662 | 397.2585 |
| 88 | 16:3-Glc-Sitosterol | C51H84O7 | 826.6555 | 397.3818 | 395.2428 |
| 89 | 18:3-Glc-Cholesterol | C51H84O7 | 826.6555 | 369.3505 | 423.2741 |
| 90 | 16:1-Glc-Stigmasterol | C51H86O7 | 828.6712 | 395.3662 | 399.2741 |
| 91 | 16:2-Glc-Sitosterol | C51H86O7 | 828.6712 | 397.3818 | 397.2585 |
| 92 | 18:2 Glc-Cholesterol | C51H86O7 | 828.6712 | 369.3505 | 425.2898 |
| 93 | 16:0-Glc-Stigmasterol | C51H88O7 | 830.6868 | 395.3662 | 401.2898 |
| 94 | 16:1-Glc-Sitosterol | C51H88O7 | 830.6868 | 397.3818 | 399.2741 |
| 95 | 18:1-Glc-Cholesterol | C51H88O7 | 830.6868 | 369.3505 | 427.3054 |
| 96 | 16:0-Glc-Sitosterol | C51H90O7 | 832.7025 | 397.3818 | 401.2898 |
| 97 | 18:0-Glc-Cholesterol | C51H90O7 | 832.7025 | 369.3505 | 429.3211 |
| 98 (I.S.) | 16:0-Glc-Stigmastanol | C51H92O7 | 834.7181 | 399.3975 | 401.2898 |
| 99 | 18:3-Glc-Campesterol | C52H86O7 | 840.6712 | 383.3662 | 423.2741 |
| 100 | 18:2-Glc-Campesterol | C52H88O7 | 842.6868 | 383.3662 | 425.2898 |
| 101 | 18:1-Glc-Campesterol | C52H90O7 | 844.7025 | 383.3662 | 427.3054 |
| 102 | 18:0-Glc-Campesterol | C52H92O7 | 846.7181 | 383.3662 | 429.3211 |
| 103 (I.S.) | 18:0-Glc-Campestanol | C52H94O7 | 848.7338 | 385.3818 | 429.3211 |
| 104 | 18:3-Glc-Stigmasterol | C53H86O7 | 852.6712 | 395.3662 | 423.2741 |
| 105 | 18:3-Glc-Sitosterol | C53H88O7 | 854.6868 | 397.3818 | 423.2741 |
| 106 | 18:2-Glc-Stigmasterol | C53H88O7 | 854.6868 | 395.3662 | 425.2898 |
| 107 | 20:3-Glc-Cholesterol | C53H88O7 | 854.6868 | 369.3505 | 451.3054 |
| 108 | 18:2-Glc-Sitosterol | C53H90O7 | 856.7025 | 397.3818 | 425.2898 |
| 109 | 18:1-Glc-Stigmasterol | C53H90O7 | 856.7025 | 395.3662 | 427.3054 |
| 110 | 20:2-Glc-Cholesterol | C53H90O7 | 856.7025 | 369.3505 | 453.3211 |
| 111 | 18:0-Glc-Stigmasterol | C53H92O7 | 858.7181 | 395.3662 | 429.3211 |
| 112 | 18:1-Glc-Sitosterol | C53H92O7 | 858.7181 | 397.3818 | 427.3054 |
| 113 | 20:1-Glc-Cholesterol | C53H92O7 | 858.7181 | 369.3505 | 455.3367 |
| 114 | 18:0-Glc-Sitosterol | C53H94O7 | 860.7338 | 397.3818 | 429.3211 |
| 115 | 20:0-Glc-Cholesterol | C53H94O7 | 860.7338 | 369.3505 | 457.3524 |
| 116 (I.S.) | 18:0-Glc-Stigmastanol | C53H96O7 | 862.7494 | 399.3975 | 429.3211 |
| 117 | 20:3-Glc-Campesterol | C54H90O7 | 868.7025 | 383.3662 | 451.3054 |
| 118 | 20:2-Glc-Campesterol | C54H92O7 | 870.7181 | 383.3662 | 453.3211 |
| 119 | 20:1-Glc-Campesterol | C54H94O7 | 872.7338 | 383.3662 | 455.3367 |
| 120 | 20:0-Glc-Campesterol | C54H96O7 | 874.7494 | 383.3662 | 457.3524 |
| 121 | 20:3-Glc-Stigmasterol | C55H90O7 | 880.7025 | 395.3662 | 451.3054 |
| 123 | 22:3-Glc-Cholesterol | C55H92O7 | 882.5773 | 369.3505 | 479.3367 |
| 124 | 20:2-Glc-Stigmasterol | C55H92O7 | 882.7181 | 395.3662 | 453.3211 |
| 125 | 20:3-Glc-Sitosterol | C55H92O7 | 882.7181 | 397.3818 | 451.3054 |
| 126 | 20:1-Glc-Stigmasterol | C55H94O7 | 884.7338 | 395.3662 | 455.3367 |
| 127 | 20:2-Glc-Sitosterol | C55H94O7 | 884.7338 | 397.3818 | 453.3211 |
| 128 | 22:2-Glc-Cholesterol | C55H94O7 | 884.7338 | 369.3505 | 481.3524 |
| 129 | 20:0-Glc-Stigmasterol | C55H96O7 | 886.7494 | 395.3662 | 457.3524 |
| 130 | 20:1-Glc-Sitosterol | C55H96O7 | 886.7494 | 397.3818 | 455.3367 |
| 131 | 22:1-Glc-Cholesterol | C55H96O7 | 886.7494 | 369.3505 | 483.3680 |
| 132 | 20:0-Glc-Sitosterol | C55H98O7 | 888.7651 | 397.3818 | 457.3524 |
| 133 | 22:0-Glc-Cholesterol | C55H98O7 | 888.7651 | 369.3505 | 485.3837 |
| 134 | 22:3-Glc-Campesterol | C56H94O7 | 896.5929 | 383.3662 | 479.3367 |
| 135 | 22:2-Glc-Campesterol | C56H96O7 | 898.7494 | 383.3662 | 481.3524 |
| 136 | 22:1-Glc-Campesterol | C56H98O7 | 900.7651 | 383.3662 | 483.3680 |
| 137 | 22:0-Glc-Campesterol | C56H100O7 | 902.7807 | 383.3662 | 485.3837 |
| 138 (I.S.) | 22:0-Glc-Campestanol | C56H102O7 | 904.7964 | 385.3818 | 485.3837 |
| 139 | 22:3-Glc-Stigmasterol | C57H94O7 | 908.5929 | 395.3662 | 479.3367 |
| 140 | 22:3-Glc-Sitosterol | C57H96O7 | 910.6086 | 397.3818 | 479.3367 |
| 141 | 22:2-Glc-Stigmasterol | C57H96O7 | 910.7494 | 395.3662 | 481.3524 |
| 142 | 22:1-Glc-Stigmasterol | C57H98O7 | 912.7651 | 395.3662 | 483.3680 |
| 143 | 22:2-Glc-Sitosterol | C57H98O7 | 912.7651 | 397.3818 | 481.3524 |
| 144 | 22:0-Glc-Stigmasterol | C57H100O7 | 914.7807 | 395.3662 | 485.3837 |
| 145 | 22:1-Glc-Sitosterol | C57H100O7 | 914.7807 | 397.3818 | 483.3680 |
| 146 | 22:0-Glc-Sitosterol | C57H102O7 | 916.7964 | 397.3818 | 485.3837 |
| 147 (I.S.) | 22:0-Glc-Stigmastanol | C57H104O7 | 918.8120 | 399.3975 | 485.3837 |
I.S., internal standard.
a
a Stigmasterol and isofucosterol were not distinguished by Q-TOF measurements.
b
b Formula of the parental, non-charged molecule.
c
c Molecular weight of the cation formed of the parental molecule with ammonium.