. Author manuscript; available in PMC: 2011 Apr 11.

Published in final edited form as: J Immunol. 2010 Mar 31;184(9):5232–5241. doi: 10.4049/jimmunol.0902501

Table I.

Primer sequences and conditions for semiquantitative PCR and qPCR

Target	Product Length (bp)	Annealing Temperature (°C)	Primer (5′-3′)	Genbank Accession No. or Reference
PlGF	440 (Is1)	51	Forward: GAGGCTGTTCCCTTGCTTC	NM_002632
(sPCR)	516 (Is2)		Reverse: GGTTACCTCCGGGGAACAG
VEGF-A	100 (Is₁₂₁)	54	Forward: GTGAATGCAGACCAAAGAAAG	NM_003376
(sPCR)	210 (Is₁₆₅)		Reverse: AAACCCTGAGGGAGGCTC
	282 (Is₁₈₉)
VEGF-A₁₆₅	79	54.4	Forward: GCCTTGCCTTGCTGCTCTAC	NM_003376
(qPCR)			Reverse: TGATTCTGCCCTCCTCCTTCTG
VEGF-B	230 (Is₁₆₇)	59	Forward: TGTCCCTGGAAGAACACAGCC	NM_003377
(sPCR)	336 (Is₁₈₆)		Reverse: GCCATGTGTCACCTTCGCA
VEGF-C	197	52	Forward: ATGTTTTCCTCGGATGCTGGA	NM_005429
(s/qPCR)			Reverse: CATTGGCTGGGGAAGAGTTT
VEGF-D	226	52	Forward: GTATGGACTCTCGCTCAGCAT	NM_004469
(sPCR)			Reverse: AGGCTCTCTTCATTGCAACAG
GAPDH	141	55	Forward: GTCCACTGGCGTCTTCAC	32
(s/qPCR)			Reverse: CTTGAGGCTGTTGTCATACTTC