Table II.

Effects of metabolic inhibitors on hGX-induced release of VEGF-A and VEGF-C from HLMs

	VEGF-A (pg/mg of Protein)	VEGF-C (pg/mg of Protein)
Vehicle + hGX	650 ± 128	516 ± 79
Act-D + hGX	74 ± 18*	53 ± 23*
CHX+ hGX	70 ± 13*	56 ± 18*
BFA + hGX	64 ± 15*	45 ± 19*

HLMs were preincubated (37°C, 30 min) with vehicle, Act-D (1 μg/ml), CHX (10 μg/ml), or BFA (10 μg/ml) and then stimulated (37°C, 24 h) with hGX (3 μg/ml). VEGF-A and VEGF-C release was determined in the supernatants by ELISA and normalized for the total protein content. Data are mean ± SEM of three experiments.

^*p < 0.05 versus vehicle and hGX.