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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: J Vasc Res. 2009 Jun 30;46(6):561–571. doi: 10.1159/000226224

Figure 2. Shear stresses affect expression and activation of eNOS through PI3K/Akt signaling pathway.

Figure 2

Western blot images and quantitative measurements of proteins expressed by PAECs: (a) total eNOS (T-eNOS) expressed as the ratio of T-eNOS to β-actin; (b) phosphorylated eNOSer1177 (P-eNOS) expressed as the ratio of P-eNOS to T-eNOS; and (c) Phosphorylated AktSer473 (P-Akt) expressed as the ratio of P-Akt to total Akt (T-Akt). Protein expressions were measured with PAECs exposed to shear stresses of 0, 5, 20, 60, 90, 120 dynes/cm2. (d) Total and phosphorylated eNOS expression under flow of 20 dynes/cm2 with or without wortmannin (100nM). Data represent means ± SEM, n=4–6 for each group. The conditions marked with ‘*’ are significantly different from shear stress at 20 dynes/cm2 (P ≤ 0.05).