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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Oncogene. 2008 Jun 9;27(42):5612–5623. doi: 10.1038/onc.2008.175

Figure 1.

Figure 1

Effect of Id1 overexpression on bone marrow cells (BMC) proliferation in suspension and semisolid media. Mouse BMC were harvested 3 days after 5-FU treatment, and then transduced with MSCV-Id1-GFP (5FU-Id1) or MSCV-GFP (5FU-MSCV). (a) Sorted green fluorescent protein (GFP) positive cells were placed in semisolid agar media supplemented with cytokine combination as indicated (50 ng/ml hG-CSF, 100 ng/ml hM-CSF, 100 ng/ml mSCF, 20 ng/ml mGM-CSF, 30 ng/ml mIL-3). Colony forming units (CFU) from GFP positive cells were scored after 10 days. Data are presented as CFU per 5 × 104 BMC ± s.e.m. of duplicate plates. (b) Photographs demonstrating size and frequency of CFU; grid = 2 mm. (c) Proliferation of sorted GFP positive cells in suspension culture with or without cytokines (100 ng/ml mSCF, 50 ng/ml hIL-6, 100 ng/ml hTPO, 100 ng/ml hFlt3L) in complete Iscove’s modified Dulbecco’s medium (cIMDM). Results are representative of two separate experiments.