Skip to main content
. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: J Med Chem. 2011 Feb 3;54(5):1288–1297. doi: 10.1021/jm101248v

Figure 6. Potential mechanisms of fluorinated N,N-dialkylaminostilbenes in Wnt inhibition.

Figure 6

(A) 4s has strong fluorescence at 365 nM (detected by Promega GloMax® Luminometer). (B) 4s is an active Wnt inhibitor. (C) 4s was incubated with LS174 CRC cells for 2h, 6h, 12h, and 24h. The localization of 4s was analyzed by confocal fluorescence microscopy. (D) Immunofluorescence staining of β-catenin in LS174 cells treated with DMSO or 4r. Nuclei were stained with DAPI. (F) 4r (10 µM) and resveratrol (100 µM) reduced the protein levels of Wnt/ β-catenin targets in LS174 cells. 4r and resveratrol also reduced the protein levels of TCF4 and Pygopus2 but not β-catenin. (G) 4r repressed the transcription of Wnt target genes.