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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Nat Methods. 2011 Feb 13;8(4):327–333. doi: 10.1038/nmeth.1571

Figure 6.

Figure 6

3D PALM image up to ~7.5 microns in depth. 50 nm-thick xy slices of PA-mCherry1-lamin B1 fusions in a fixed Cos7 cell, at axial depths of 1.5- (left), 3- (middle), and 6 μm (right); scale bars 3 μm. A magnified view (inset) of the highlighted region shows a thin section of membrane resolved to ~50 nm at an axial depth of 6.58 μm (indicated by arrow); scale bar 1 μm. Only localizations with a correlation strength >0.4 are shown, bin sizes are 50 nm (main panels) and 25 nm (inset). Supplementary Video 4 steps through xy slices of this 3D image with 50 nm pixel size and 50 nm z separation, rendered from ~1.9 million unlinked localizations.