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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Cytometry A. 2011 Jan;79(1):84–89. doi: 10.1002/cyto.a.20986

Figure 4. Potential utility of the elimination of fluorescence.

Figure 4

PBMC were stained with PE-anti-CD3 and QD655 conjugated to either anti-CD4 (top) or anti-CD8 (bottom). After analysis (left), cells were treated for 30 min with 30 µM cupric sulfate and reanalyzed (middle). Finally, cells were incubated for 30 min with 1 mM EDTA and washed three times, then re-stained with QD655 conjugated to either anti-CD8 (top) or anti-CD4 (bottom).