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. 2010 Nov 10;30(45):15097–15101. doi: 10.1523/JNEUROSCI.3549-10.2010

Figure 1.

Figure 1.

Phosphorylated Trk is localized to postsynaptic densities. A, Montages of photomicrographs collected from dorsal hippocampus show DAPI labeling of nuclei (top; image intensities inverted), immunofluorescence labeling for PSD95 (green) and pTrkBY515 (red), and a merger of PSD95 and TrkBY515 immunolabeling (sr: str. radiatum; scale bar: 500 μm). The pTrkBY515 and pTrkY490 immunoreactivities have similar distributions in hippocampus (supplemental Fig. 1, available at www.jneurosci.org as supplemental material) (also, Chen et al., 2010). B, Representative deconvolution micrographs show immunofluorescence for pTrkY490 (red) and PSD95 (green) in CA3a str. radiatum. Arrows indicate double-labeled elements (yellow in “merge”). Scale bar: 10 μm. C, High magnification of a single synapse double-labeled for pTrkY490 (red) and PSD95 (green). Scale bar: 1 μm.