Figure 2.

Structure and function of postsynaptic neurons are not affected by the Df(16)1 microdeletion in mature mice. a, Representative images of the apical dendrites of CA1 neurons from slices of WT (left) and Df(16)1/+ (right) littermates. Scale bar, 50 μm. b, Scholl analysis of apical dendritic tree branching of CA1 neurons in WT and Df(16)1/+ mice. c, Representative TPLSM images of dendritic spines in CA1 neurons of WT (left) and Df(16)1/+ (right) mice. Scale bar, 2 μm. d, Mean dendritic spine density (27–28 dendrites), length, and width (87–123 randomly selected spines) on CA1 neurons from WT and Df(16)1/+ mice. e, f, Representative electron micrographs (e) and mean lengths of postsynaptic densities (f) at synapses in the CA1 areas of WT and Df(16)1/+ mice. g, h, Representative traces of AMPAR-mediated (recorded at −70 mV, downward traces) and NMDAR-mediated (recorded at +40 mV, upward traces) EPSCs (g) and the mean ratio of AMPA/NMDA currents (h) recorded at CA3–CA1 synapses from WT and mutant mice. i, Representative traces of changes in the membrane potentials in CA1 neurons in response to injection of currents of different amplitudes (−25–225 pA; increment, 25 pA). j, Average number of APs fired in response to a 75 pA depolarization current and average threshold membrane potential required for generation of APs in CA1 neurons of WT and Df(16)1/+ mice.