Figure 4.

Neurotransmitter release is enhanced in mature Df(16)1/+ mice. a, Representative images of presynaptic boutons loaded with the fluorescent dye FM 1-43 before or after synaptic stimulation (arrow). b, Examples of destaining curves for 16 boutons before and after 10 trains of synaptic stimulation (arrows). Each arrow represents 40 stimulations at 200 Hz. c, Average FM 1-43 destaining curves for boutons in slices from WT and Df(16)1/+ littermates (7 slices per genotype, 17–67 boutons per slice; *p < 0.05). d, Cumulative histograms of rates of FM 1-43 destaining in boutons from WT or Df(16)1/+ mice (242–265 boutons; *p < 0.01).