Figure 6.

The level of SERCA2 protein is elevated in the hippocampus of mature but not young Df(16)1/+ mice. a, b, Representative Western blots of hippocampal extracts from WT and Df(16)1/+ littermates aged 6 weeks (a) or 16 weeks (b). The β-actin (control) signal is within detection range of the Odyssey imager. c, Average relative hippocampal protein levels of SERCA2 in young and mature WT and Df(16)1/+ mice normalized to β-actin levels. d, Western blot showing the enrichment of the presynaptic protein synaptophysin (SPH) and the postsynaptic NMDA receptor NR2A in crude synaptosomal preparations from hippocampus. e, f, Representative lanes from Western blots (e) and quantification of SERCA2 levels in synaptosomal preparations from WT and Df(16)1/+ hippocampus (f). *p < 0.01.