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Increased buffering capacity in physiological buffer reduces mIPSC amplitude, rise time, and charge transfer. A, Raw trace from one CGC at −60 mV holding potential shows the effect of increased buffer capacity. B, Averaged traces from one cell (>100 events per condition). C, Effect of increased buffering on rise time. D, As in Figure 2 C, scatter plot of mean charge transfer values for each neuron under the two buffering conditions. Values from n = 11 neurons; *p < 0.05.
