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Glycopeptide analysis using ETD and CID-MS/MS. (A) ETD-MS/MS cleaves the peptide backbone, generating c and z• ions. (B) CID-MS/MS cleaves the glycan linkage, generating oxonium ions and glycopeptide fragments. (C) Averaged mass spectra contain all the detectable glycans on this glycopeptide. NL: normalized level. Key to symbols: empty circle = galactose, filled circle = mannose, filled square = HexNAc, filled diamond = NeuAc, filled triangle = fucose.
