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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Trends Cell Biol. 2010 Dec 21;21(3):159–167. doi: 10.1016/j.tcb.2010.11.003

Figure 2.

Figure 2

GARP localizes to the TGN, where it enables cargo transport from endosomes. (a–c) Co-localization of GARP (labeled by expression of a Vps54-GFP chimera) (green channel) with the TGN marker TGN46 (labeled with a specific antibody to this protein followed by Alexa-594 conjugated secondary antibody) (red channel) in human HeLa cells imaged by confocal fluorescence microscopy. Panel c shows a merged image in which yellow indicates co-localization. The TGN appears as a cisternal/tubular network partially surrounding the nucleus (Nu). Cy: cytoplasm. (d,e) Evidence for the involvement of GARP in retrograde transport. Confocal fluorescence microscopy shows that internalized Cy3-conjugated B subunit of Shiga toxin (STxB) reaches the TGN in control HeLa cells, but accumulates in endosomes and retrograde transport carriers in HeLa cells depleted of Vps51 (i.e., Ang2) by RNAi knock-down (KD). Images are reproduced from refs. [13] (a–c) and [18] (d,e).