Skip to main content
. 2011 Mar 3;66(5):985–996. doi: 10.1093/jac/dkr044

Figure 3.

Figure 3.

Formation of ELB-21 intrastrand adducts with dsDNA 13-mer duplex-2. Ion-pair RPLC chromatograms of (a) duplex-2, showing denaturation to Seq-3 (RT 27.1 min) and Seq-4 (RT 28.2 min) under RPLC conditions; (b) immediately (0 h) after mixing of ELB-21 with duplex-2, showing the rapid appearance of intrastrand 5′-Pu-GAATG-Py adduct (RT 29.8 min). Seq-3, which contains the 5′-Pu-GAATG-Py intrastrand binding site, has almost disappeared; (c) 1 h after mixing of ELB-21 with duplex-2, showing emergence of two minor peaks (RT 32.4 min and RT 36.3 min) in addition to a major peak (RT 29.8 min). Seq-3 is completely absent, indicating completion of the reaction; (d) 12 h after mixing of ELB-21 with duplex-2. Peaks were characterized using MALDI-TOF MS.