Table 1.
Primers sequences for RT-PCR
| Gene name | 5′- Sequence -3′ | Product size (bp) | GenBank Number | |
|---|---|---|---|---|
| β-actin | Forward Reverse |
AAGTACCCCATTGAACACGG ATCACAATGCCAGTGGTACG |
257 | NM_031144 |
| Bmal1 | Forward Reverse |
CCAAGAAAGTATGGACACAGACAAA GCATTCTTGATCCTTCCTTGGT |
81 | BC025973 |
| Clock | Forward Reverse |
CAAAATGTCACGAGCACTTAATGC ATATCCACTGCTGGCCTTTGG |
84 | MMAF000998 |
| Per1 | Forward Reverse |
AAACCTCTGGCTGTTCCTACCA AATGTTGCAGCTCTCCAAATACC |
74 | NM_011065 |
| Per2 | Forward Reverse |
ATGCTCGCCATCCACAAGA GCGGAATCGAATGGGAGAAT |
72 | NM_011066 |