Table 7.14.1.

Water activity (lna_W) of co-solute buffers^a

Buffer	Co-solute concentration g/mLb	lnaW
CNE 0.3 onlyc	-	0.0104
5% ethylene glycol in CNE 0.3	0.0516	0.0248
10% ethylene glycol in CNE 0.3	0.1002	0.0392
15% ethylene glycol in CNE 0.3	0.1510	0.0541
20% ethylene glycol in CNE 0.3	0.2078	0.0665
5% glycerol in CNE 0.3	0.0508	0.0204
10% glycerol in CNE 0.3	0.0992	0.0311
15% glycerol in CNE 0.3	0.1499	0.0433
20% glycerol in CNE 0.3	0.1998	0.0569
5% acetamide in CNE 0.3	0.0500	0.0250
10% acetamide in CNE 0.3	0.1006	0.0399
15% acetamide in CNE 0.3	0.1510	0.0551
20% acetamide in CNE 0.3	0.2026	0.0686

^aThe water activities were determined experimentally by Spink and Chaires (Spink and Chaires 1999).

^bSolutions were prepared by directly weighing the precise amount of co-solute in a volumetric flask and diluting to desired volume with CNE 0.3 buffer.

^c10 mM sodium cacodilate (pH 7.4), 300 mM NaCl and 0.1 mM EDTA