Table 7.14.2.
Experimental UV thermal melting data of 5’-r(MeU2’-FpApMeU2’-FpApMeU2’-FpApMeU2’-FpApMeU2’-FpApMeU2’-FpA) in acetamide buffers.
Acetamide in CNE 0.3 buffer | Derivative α curve | Van't Hoff plot | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Experiment | 0% | 5% | 10% | 15% | 20% | T1 | T2 | ΔH cal/mol |
ΔH cal/mol |
ΔS eu |
es050a | 55.4 | 52.3 | 50.8 | 48.5 | 47.4 | 49.02 | 62.45 | 81559 | 86700 | 237.8 |
es050b1 | 55.3 | 52.4 | 50.9 | 48.3 | 47.2 | 49.01 | 62.26 | 82618 | 85700 | 235.1 |
es050b2 | 55.3 | 52.2 | 50.8 | 48.4 | 47.1 | 49.17 | 62.30 | 83424 | 85600 | 234.5 |
es050b3 | 55.1 | 52.2 | 50.9 | 48.5 | 47.2 | 49.08 | 62.30 | 82833 | 74500 | 201.1 |
es050c1 | 55.3 | 52.1 | 50.9 | 48.5 | 46.7 | 49.11 | 62.14 | 84009 | 85900 | 235.6 |
es050c2 | 55.1 | 52.1 | 50.8 | 48.7 | 47.0 | 48.92 | 62.37 | 81393 | 72000 | 192.9 |
es050d | 55.2 | 52.0 | 50.8 | 48.5 | 47.4 | 48.91 | 62.33 | 81563 | 74400 | 200.5 |
es050e1 | 55.3 | 52.4 | 50.9 | 48.6 | 47.3 | 48.99 | 62.43 | 81486 | 79700 | 216.5 |
es050e2 | 55.3 | 52.5 | 50.9 | 48.6 | 47.4 | 48.94 | 62.97 | 78173 | 80100 | 217.6 |
es050e3 | 55.5 | 52.5 | 51.0 | 48.7 | 47.5 | 48.95 | 62.88 | 78715 | 78700 | 213.3 |
es050f1 | 55.5 | 52.7 | 51.0 | 48.6 | 47.5 | 49.09 | 62.54 | 81477 | 78200 | 211.9 |
es050f2 | 55.7 | 52.6 | 50.9 | 48.7 | 47.3 | 48.99 | 63.08 | 77878 | 81200 | 220.8 |
es050f3 | 55.6 | 52.5 | 50.8 | 48.6 | 47.4 | 49.27 | 62.86 | 80760 | 79300 | 215.6 |
Average | 55.4 | 52.3 | 50.9 | 48.6 | 47.3 | 48.8 | 62.5 | 80194 | 81200 | 221.4 |
Stand. Dev. | 0.2 | 0.2 | 0.1 | 0.1 | 0.2 | 0.4 | 0.3 | 2937 | 4100 | 12.5 |