Table 1. Peripheral blood cell number and percent of T cells, B cells, and T cell subsets in the peripheral blood of Npsr1-deficient mice.
Normal blood cell composition was assessed in the Npsr1-deficient (KO) and wildtype (WT) mice (n = 8 for each genotype). WBC, White Blood Cells; NE, Neutrophils; LY, Lymphocytes; MO, Monocytes; EO, Eosinophils; BA, Basophils; RBC, Red blood cells; HGB, Hemoglobin; PLT, Platelets. Flow cytometry was used to analyze T cells, B cells and T cell subtypes in the peripheral blood from WT and KO mice (n = 3 for each genotype) using B220, CD3, CD4, and CD8 markers. These results are representative of three independent experiments. Data represent the mean ± SEM.
| WBC (K/uL) |
NE (K/uL) |
LY (K/uL) |
MO (K/uL) |
EO (K/uL) |
BA (K/uL) |
RBC (M/uL) |
|
|---|---|---|---|---|---|---|---|
| WT | 6.47±0.65 | 1.51±0.16 | 4.59±0.53 | 0.33±0.06 | 0.03±0.01 | 0.01±0.01 | 8.39±0.36 |
| KO | 7.94±0.67 | 1.68±0.20 | 5.86±0.48 | 0.36±0.04 | 0.04±0.02 | 0.01±0.01 | 8.87±0.34 |
| HGB (g/dL) |
PLT (K/uL) |
CD3 (%) |
B220 (%) |
CD4 (%) |
CD8 (%) |
|
|---|---|---|---|---|---|---|
| WT | 13.96±0.49 | 647.38±45.79 | 34±4.73 | 34±3.06 | 27±2.96 | 14±2.40 |
| KO | 14.24±0.53 | 654.13±28.51 | 30±3.18 | 28±3.00 | 26±2.96 | 11±1.53 |
Data expressed as mean ± SEM (n=8 for blood cell counting and n=3 for FACS)