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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Oral Microbiol. 2011 Jan 27;26(2):127–139. doi: 10.1111/j.2041-1014.2010.00603.x

Figure 1.

Figure 1

RNA oligonucleotide quantification technique (ROQT) membrane used to assess probe sensitivity. Total nucleic acids extracted from selected bacterial species were used as targets in the horizontal lanes. Oligonucleotide probes in the vertical lanes were hybridized against these targets. (A) Targets at 106 to 103 bacterial cells. because of higher than ideal concentration of the Campylobacter rectus probe, signals became saturated for both cell concentrations. (B) Targets at 105 to 104 bacterial cells.