Figure 10. Apoptosis (TUNEL stain, green) in combination with recoverin stain (red), comparison of line-3 (A,C) with line-5 (B,D).
(A) S334ter-3 rat at P11. Numerous TUNEL+ cells in ONL. (B) S334ter-5 rat at P13: Strong TUNEL stain in ONL, but less cells than in line 3. (C) S334ter-3 rat at P30: strong TUNEL stain in remnant of ONL. ONL reduced to 2–3 layers of nuclei. Faint stain of some cells in INL and GCL. (D) S334ter-5 rat at P30: Thicker ONL (5 layers of nuclei) - strong TUNEL stain of cells in ONL. In addition, some cells in GCL are also strongly TUNEL stained. Fainter stain of cells in INL and GCL. Magnification bars = 20 μm